Figure 2.

Spliceosome dynamics and retinopathy. The assembly/disassembly cycle of the spliceosome (adapted with permission from Wahl et al. 2009) during splicing with only the pre-mRNA (blue and yellow boxes, exons; thick black line, intron) and snRNP (grey) complexes illustrated to highlight the roles of U4-associated (PRPF3/hPrp3, PRPF31/hPrp31) and U5-associated (PRPF8/hPrp8, SNRP200/hBrr2) proteins as well as several other steps that require the DExD/H-type RNA-dependent ATPase/helicase. Steps 1 and 2 refer to the first and second catalytic steps of splicing.