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Results of PCR analysis of Chlamydia trachomatis EB in synovial samples following DNA extraction by (a) alkaline lysis and (b) Chelex®. On the y-axes concentration of Chlamydia trachomatis elementary bodies (EB)/ml are given. Each point on the graph indicates the detection limit of one serial dilution analysis. Numbers in boxes represent lowest reproducible detection limit of Chlamydia trachomatis EB/ml in synovial fluid.
