Table 4.
PCR sensitivities of the different DNA extraction methods detection Chlamydia trachomatis EB and C. trachomatis PBMO DNA/ml SF using PCR-system 1 for amplification immediately after extraction and post storage at -20°C for four months
| Sensitivity achieved with PCR amplification system 1 | 1 Alkaline lysis immediately | 1 Alkaline lysis ps | 2 Qiaex II gel extraction kit® +CTAB immediately | 2 Qiaex II gel extraction kit® + CTAB ps | 4 Qiagen Tissue Kit® immediately | 4 Qiagen Tissue Kit® Ps | 5 Qiagen Stool Kit® immediately | 5Qiagen Stool Kit® ps |
|---|---|---|---|---|---|---|---|---|
| (C. trachomatis EB/ml SF) | (0.1 -10) M 1 |
10 (1-10) M 10 |
0.1 | 1000 (1000) M 1000 |
1 (1-10) M 1 |
10 (1-10) M 10 |
1 (1-100) M 100 |
1 (1-10) M 10 |
| Statistical analysis ps | See Table 3 | * | See Table 3 | * | See Table 3 | See Table 3 | ||
| (C. trachomatis PBMO/ml SF) | (0.1-1) M 0.1 |
1000 (1000) M 1000 |
10 (0.1 - 100) M 10 |
1000 (1000) M 1000 |
10 (1-100) M 10 |
1000 (1000) M 1000 |
1 (0.1-1) M 1 |
1000 (1000) M 1000 |
| Statistical analysis ps | See Table 4 | * | See Table 4 | * | See Table 4 | * | See Table 4 | * |
Statistical analysis post storage (ps) compared with sensitivity results compared with sensitivity achieved after immediate PCR analysis are indicated by *. EB = elementary bodies; M = median; PBMO = peripheral blood monocytes; SF = synovial fluid.