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Immunocytochemistry for IL-6 and TNFα expression in co-cultures of RA SM/Jurkat. Immunocytochemistry in co-cultures of rheumatoid arthritis synovial macrophages (RA SM)/Jurkat, (a) untreated (controls) and (a1) treated with CTLA4-Ig 500 μg/ml for IL-6 and (b) untreated (controls) and (b1) treated with CTLA4-Ig 500 μg/ml for TNFα. Quantification (mean value ± standard deviation) of immunocytochemistry (ICC-QWin) for (c) IL-6 and (d) TNFα. * P < 0.05; ** P < 0.01; *** P < 0.001.
