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. 2009 Nov 9;11(6):R167. doi: 10.1186/ar2854

Figure 6.

Figure 6

TCRVβ usage of altered peptide ligand-specific CD4+ T cells. TCRVβ usage of altered peptide ligand (APL)-specific CD4+ T cells was not remarkably shifted to TCRVβ8.1 8.2. Mice were immunized with 10 μg each APL, and draining lymph node cells on days 7 to 9 were stimulated with 20 μM corresponding APL in vitro. Ratios of TCRVβ repertoire to CD4+ T cells. The TCRVβ repertoire of CD4+ T cells was analyzed by flow cytometry: before stimulation with the corresponding in vitro for 72 hours, and after stimulation.