Fig. 3.

Characterization of products of Man-P-Dol synthase reactions by thin layer chromatography. The [³H]mannolipid products of the Man-P-Dol synthase activities from rer2Δ cells (A), cells transformed with the empty plasmid (B), uppS (C), or RER2 (D) were resolved by TLC on Baker Si-250 silica plates developed in CHCl₃/CH₃OH/H₂O (75:25:4) and detected by a Bioscan AR2000 Imaging Scanner (Bioscan, Washington, DC). The migration of standard Man-P-(55)Dol, Man-P-(75)Dol, and Man-P-(95)Dol is indicated by the arrows.