Table V.
Transfer of [3H]mannose from GDP-[3H]Man into [3H]Man-P-(55/95)Dol in microsomal fractions from CHO-K1 and CHO cells expressing UPPS
| Microsomes from | [3H]Man-P-(55)Dol
(pmol/mg/min) |
[3H]Man-P-(95)Dol
(pmol/mg/min) |
|---|---|---|
| CHO-K1 | n.d. | 1.4 |
| CHO/UPPS | 1.9 | 1.8 |
Microsomal fractions were prepared from either CHO-K1 or CHO/UPPS cells and assayed for the transfer of [3H]mannose from GDP-[3H]Man into [3H]Man-P-(55/95)Dol described in “Materials and methods”. The two mannolipids were resolved as in Figure 3. The data are average values of duplicate analyses and are representative of at least two separate experiments. n. d., not detected.