Table VI.
Effect of aqueous phenol on [3H]mannolipid product synthesized from GDP-[3H]Man in microsomal fractions from CHO-K1 and CHO cells expressing UPPS
| Mannolipid synthesized by | [3H]Mannolipid hydrolyzed
(%) |
|---|---|
| CHO-K1 microsomes | 8.1 |
| CHO/UPPS microsomes | 9.4 |
| Man-P-Dol standard | 7.8 |
| Man-P-Und standard | 81.5 |
a
[3H]Mannose-labeled mannolipid products were prepared from microsomal fractions from either CHO-K1 or CHO/UPPS cells and incubated at 68°C in 50% phenol as described by Parodi and Quesada-Allue (1982). The data are average values of duplicate analyses.