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. Author manuscript; available in PMC: 2010 Dec 17.
Published in final edited form as: Biochem J. 2010 Dec 15;433(1):205–213. doi: 10.1042/BJ20101322

Figure 1. Specificity of acetylation of eIF5A(Lys) and eIF5A(Hpu) by different acetyltransferases in vitro.

Figure 1

The acetyltransferase assays were carried out as described in “Experimental”, using [3H]AcCoA, 0.05 μg of each enzyme as indicated, with 2 μg each of (A) recombinant eIF5A(Lys) or (B) recombinant eIF5A(Hpu). (C) eIF5A (hypusine form) purified from Chinese hamster ovary (CHO) cells, human red blood cells (hRBC), or rabbit reticulocyte, eIF5A(Dhp) prepared by an in vitro DHS reaction, or eIF5A(Lys) were used as substrates for SSAT1 under the same condition as in A and B, except that 0.1 μg of enzyme was used. After 1 h incubation, radioactivity incorporated into eIF5A protein was measured in the TCA-precipitated proteins and also by fluorography after SDS-PAGE. Aliquots of reaction mixtures were used for western blot analyses with AcLys antibody or eIF5A antibody. The experiments were carried out two times with similar results and representative data are shown. Abbreviation: 5A, eIF5A