Fig. 2.

Growth curves of human epithelial corneal line cells (10.014pRSV-T) after incubation with topical glaucoma medications. The cells were cultured in the absence or presence of different concentrations of medications in 8-chamber slides, stained with FAM-VAD-FMK and PI at 24 h intervals, and subsequently fixed and analyzed by LSC. Top panels show changes in cell number per culture at given point of culture harvesting. The LSC images of cells treated with brinzolamide for 24 h are shown in the bottom panels. The cells with necrotic appearance are characterized by weak green fluorescence, apoptotic by strong green cytoplasmatic fluorescence and live cells only by nuclear red fluorescence of PI. Cell incubations with 1:10 diluted medications induced necrosis, which was associated with the greater cell attachment to slides than that of apoptotic cells, which predominated in cultures treated with 1:100 diluted medications. [Color figure can be viewed in the online issue, which is available at www.interscience.wiley.com.]