Fig. 4.

Viability of the untreated and drug-treated corneal cells assessed by PI exclusion test. Corneal line cells (10.014pRSV-T) were incubated for 15 min with different concentration of brimonidine (top panels) or timolol (bottom panels) and then for 1 min with PI. The chambers were then removed, slides were covered by cover glasses and the red fluorescence was examined by confocal microscopy (200×) combined with the Nomarski interference contrast; necrotic and late apoptotic cell nuclei are stained with PI. [Color figure can be viewed in the online issue, which is available at www.interscience.wiley.com.]