Figure 5.

Effects of bafilomycin on C-DIM-induced responses. [A] Growth inhibition in breast cancer cells. MDA-MB-231 and MDA-MB-453 cells were treated with DMSO, C-DIMs alone (10 μm), or in combination with bafilomycin (150 nM) for 48 hr, and cells were counted as described in the Materials and Methods. Results are expressed as means ± SD for replicate (3) determinations. Effects of bafilomycin on LC3b protein in MDA-MB-231 [B] and MDA-MB-453 [C] cells. Cells were treated with DMSO, C-DIMs alone, or in combination with bafilomycin for 48 hr, and whole cell lysates were analyzed by western blots as outlined in the Materials and Methods.