Table 1.

Characteristics of adaptive substitutions in the D promoter of ϕX174 that arose in previous evolution experiments.

Strain	Mutation	Change in Protein C	# of Exps	Host*	Temperature, °C*
mut319	G319T	V63F	4**	Salmonella typhimurium	43.5
			2, 1	Escherichia coli	37, 43.5
mut321	T321C	none	1	Salmonella typhimurium	43.5
mut323	A323G	N64G	3	Salmonella typhimurium	43.5
			1	Escherichia coli	43.5
mut324	C324T	none	1	Shigella sonnei	42
			1	Escherichia coli	43.5

*Conditions under which substitutions arose [HA Wichman, pers. comm.; [35-38]]

** Also, one reversion after switch to E. coli host at 43.5°C.