Figure 5.
3-D structure of PvDBPII. Space-filling model of the 3-D structure PvDBPII showing the DARC-binding residues (Tyr 94, Asn 95, Lys 96, Arg 103, Leu 168 and Ile 175 - yellow) [53]. (A) Polymorphic residues identified in the Brazilian isolates are shown in blue or red for residues close to or not close to the DARC-binding domain, respectively. All positions of these residues on the sequence are indicated. (B) Residues on the 3-D structure are color coded according to the posterior probability of positive selection as calculated using omegaMap. Green residues indicate high posterior probability (HPD > 93%) of positive selection. Codons that seem to be less affected by natural positive selection (HPD < 93%) are colored in white. Residues in dark gray were not analyzed by omegaMap. (C) The previously identified epitopes 45, 48, and 66 [30,49] and the in silico predicted T-cell epitopes IIb, IIa and Ia are shown in violet. The model's rotation in the vertical plane is indicated by arrows.