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. 2010 Dec 17;5(12):e15263. doi: 10.1371/journal.pone.0015263

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Marim et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Bone marrow-derived dendritic cells (BMDCs) obtained from fresh or cryopreserved (frozen) bone marrow cells were either left untreated or stimulated with 1 µg/ml LPS for 24 h. BMDCs were stained for CD11b, CD11c, CD40, CD86 and MHC-II and analyzed by flow cytometry. Plots were gated on CD11b⁺ cells. Shown are the percentages (A) and mean fluorescence intensities (B) of CD11c⁺ cells expressing CD40, CD86 or MHC-II in response to LPS treatment. Data are representative of those found in two independent experiments. No statistically significant differences were detected between BMDCs obtained from fresh or frozen BM cells (P>0.05).