Figure 1.
Effect of gallic acid on cell viability and proliferation of glioma cells. A: MTT assay of U87 and U251n glioma cells treated with various concentrations of gallic acid. B: SRB assay of U87 and U251n glioma cells treated with the same concentrations. C: U87 and U251n were treated with the indicated concentration of gallic acid for up to 96 h in complete medium. MTT assay was used to assess growth of cells in culture. D: BrdU proliferation assay of U87 and U251n cells treated with different doses of gallic acid for 24 h. The cell nuclear incorporation of BrdU was measured. The proliferation rate was presented as mean ± SEM value of percentage from BrdU-labeled cells vs. DAPI-labeled cells (data obtained from three independent experiments). *P<0.01, control vs. treated groups.