Fig. 9.

The N-terminal domain mediates FCRLA intracellular retention. (A) Model of FCRLA showing its domain structure. (B and C) 293T cells were transfected with cDNA expression vectors encoding the indicated FCRLA deletion mutants. (B) Two days after transfection, lysate of cells (Lys) and supernatants of conditioned media (Sup) were subjected to immunoblotting with FCRLA antibody. (C) Deglycosylation of cell lysates of wild-type FCRLA and the FCRLAΔd12 deletion mutant. Not treated (n.t.) and deglycosylated (deglyco) cell lysates.