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. Author manuscript; available in PMC: 2011 Dec 17.
Published in final edited form as: ACS Chem Biol. 2010 Oct 5;5(12):1147–1155. doi: 10.1021/cb100195d

Figure 2.

Figure 2

Figure 2

Observation and measurement of incorporated fluorescence into wild-type or SrtA-knockout S. aureus following small molecule treatment. (A) Synthetic small molecules employed in these studies. (B) Epifluorescence microscopy. Abbreviations: “wt” indicates wild-type S. aureus, “SrtA KO” indicates a Sortase A knockout strain of S. aureus,(16) “Fluor” indicates epifluorescence images, and “Merge” corresponds to fluorescent images superimposed atop brightfield images. All micrographs were acquired under identical exposure settings, and processed in an identical fashion. The depicted trends were reproduced on at least three separate occasions. (C) Quantitation of fluorescein-incorporation via flow cytometry.