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. Author manuscript; available in PMC: 2011 Dec 17.
Published in final edited form as: ACS Chem Biol. 2010 Oct 5;5(12):1147–1155. doi: 10.1021/cb100195d

Figure 3.

Figure 3

SrtA-targeted small molecules are localized to the cell wall. (A) Fractionation of wild-type S. aureus cells into cell wall and cytosolic extracts following treatment with compounds 1 or 2. Data represent the average of triplicate experiments ± SD. (B) Immunocryoelectron microscopy studies of a protein A-knockout S. aureus strain in the Newman genetic background. Cells were incubated in the presence (panel a) and absence (panel b) of 1. Arrow points to an example of black dots, which correspond to gold-labeled antibody binding. Abbreviations: anti-FITC = rabbit anti-fluorescein antibody; Au-secondary = gold-labeled anti-rabbit secondary antibody.