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. 2010 Mar 17;28(1):145–158. doi: 10.1007/s11095-010-0093-y

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© The Author(s) 2010

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Fig. 4 — DT-specific IgG titers and neutralizing antibody titers induced by DT liposome formulations in TCI. Formulations were applied occlusively on microneedle-treated skin (unless specified in a) at day 0, 21 and 42; s.c. injection of DT-alum as control. Sera were collected after prime, the first boost and the second boost (day 20, 41 and 55), and IgG titers were determined with ELISA (a and b). Data shown as mean + SD (n = 8). Non-responders were given an arbitrary log-value of 1. Neutralizing antibody titers after the second boost were evaluated with Vero cell test (c). An arbitrary log-value of 0 was given when titers were below detection limit (*: p < 0.05, **: p < 0.01, ***: p < 0.001).