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. 2010 Sep 16;62(2):497–508. doi: 10.1093/jxb/erq283

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© 2010 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 3. — Expression of AtTSPO in yeast reduces cell growth. (A, C) Yeast strains (EV, 6×His AtTSPO, AtTSPO-6×His) growth monitored by OD₆₀₀ nm absorbance either in SD-glucose medium (A) or SD-galactose medium (C). (B, D) The same yeast strains growth was also monitored through a serial dilution drop test; lower panels in (B) and (D) show the detection by Western blot of AtTSPO expression after growth in SD-glucose (B) or SD-galactose (D). EV, 6×His-AtTSPO, and AtTSPO-6×His stand for transgenic yeast transformed with, respectively, the empty vector, expression vector containing N-terminal tagged version of AtTSPO, and the expression vector containing C-terminal tagged version of AtTSPO. The filled squares are for the EV strain, the filled circles for 6×His-AtTSPO, and the open circles for AtTSPO-6×His.