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. 2010 Sep 16;62(2):497–508. doi: 10.1093/jxb/erq283

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© 2010 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 5. — Enrichment of AtTSPO in purified yeast microsomal fractions. TM stands for total microsomes; the ratio values >1 indicates an enrichment, and <1 indicates impoverishment with respect to the TM. (A) Immunoblot analysis of the mitochondria-enriched fraction (M) prepared from the strains expressing either the N-terminal tagged version of AtTSPO or the C-terminal tagged version of AtTSPO; anti-VDAC1 is used as a positive control. (B) Immunoblot analysis of the plasma membrane enriched fraction (P) prepared from the AtTSPO expressing yeast strains 6×His-AtTSPO and AtTSPO-6×His and the strain transformed with the empty vector, EV. Anti-Gas1 protein is used as a positive control for plasma membrane.