Fig. 7.

AtTSPO and VDAC1 interact physically. VDAC1 specific antibodies (A) or AtTSPO specific antibody (B) were used to generate an immunocomplex (IP) of their respective antigen from total protein extracts from yeast transformed with the empty vector (EV) or 6×His-AtTSPO. The immunocomplexes were coupled to protein A beads; the input (IN), flowthrough (FT), the last wash (W), and the eluted fraction (E) were analysed by immunoblotting (IB). To verify the specificity of the interactions, a check was made for the presence of Gas1p, a plasma membrane protein, in the immunocomplexes.