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. 2010 Oct 13;62(2):605–615. doi: 10.1093/jxb/erq294

Fig. 6.

Fig. 6.

Changes in expression of genes related to glutamate metabolism. Total RNA was isolated from axes of Medicago truncatula subjected to H2O (control), PEG (–0.25 MPa) or ABA (10 μM). Each RNA sample was used for quantitative RT-PCR analyses. The cDNA copy numbers for glutamine synthetase (GS1b and GS2), glutamate dehydrogenase (GDH3), NADH-GOGAT, asparagine synthetase (AS), and Δ1-pyrroline-5-carboxylate synthetase (P5CS) gene were expressed as ratio (treatment versus H2O). Each datum point is the mean of three independent experiments ±SE.