Fig. 4.

NaBAK1 positively regulates wounding- and herbivory-induced accumulation of JA and JA-Ile. Leaves of EV and NaBAK1-VIGS plants were wounded with a pattern wheel and 20 μl of water (W+W), M. sexta oral secretions (W+OS), or FAC A solution (W+FAC) were immediately applied to each leaf. Individual leaves from five replicate plants were harvested after 1.5 h. Mean JA (A) and JA-Ile (B) concentrations (±SE) in EV and NaBAK1-VIGS plants were analysed with HPLC-MS/MS. Different letters indicate significant differences (two-way ANOVA, Fisher's PLSD test; P <0.05; n=5).