Fig. 5.

Transcript accumulation of NaTD, NaJAR4, and NaJAR6 in wounding- and herbivory-induced EV and NaBAK1-VIGS plants. Leaves of EV and NaBAK1-VIGS plants were wounded with a pattern wheel and 20 μl of water (W+W) or M. sexta oral secretions (W+OS) were immediately applied to each leaf. Leaves from replicate plants were harvested at the indicated times. Mean transcript levels (±SE) of N. attenuata NaTD (A), NaJAR4 (B), and NaJAR6 (C) were measured with quantitative real-time PCR. Asterisks represent significantly different levels between EV and NaBAK1-VIGS plants at the indicated times (unpaired t test; *P <0.05; **P <0.01; ***P <0.001; n=5).