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Silencing NaBAK1 does not impair wounding- and herbivory-induced MAPK activity in N. attenuata leaves. Leaves of EV and NaBAK1-VIGS plants were treated with W+W or W+OS and harvested at the indicated times. MAPK activity was analysed by an in-gel kinase assay using myelin basic protein as the substrate (upper panel). Same amount of each protein sample was run on another PAGE gel and stained with Coomassie Brilliant Blue (CBB) to confirm equal loading (bottom panel).
