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. 2010 Oct 29;62(2):761–773. doi: 10.1093/jxb/erq307

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© 2010 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — miR396 negatively regulates cell proliferation by controlling entry into the mitotic cell cycle. (A and B) In situ hybridization analysis of histone H4 expression in shoot apices of 13-day-old wild-type (A) and 35S:miR396a (B) seedlings. (C) The number of histone H4-positive cells in leaves of the 13-day-old wild-type and 35S:miR396a seedlings. The asterisk indicates a statistically significant difference by t-test (P <0.05). (D) Expression of cell cycle-related genes in 13-day-old wild-type and 35S:miR396a seedlings. (E–G) Ploidy level distribution analysis. The ∼8-day-old leaves from 13-day-old seedling of the wild type, 35S:miR396a, and atgif1/an3 were used to examine the cell nuclear ploidy levels (E). The percentages of cells with different nuclear ploidy levels (F) and the ratio of 4C/2C cells (G) were calculated. Bars=100 μm in A and B. (This figure is available in colour at JXB online.)