TABLE 3.
Gene | Forward primer | Reverse primer |
Cyp1a1 | 5′-CAGACCTCAGCTGCCCTATC-3′ | 5′-CTTGCCCAAACCAAAGAGAG-3′ |
Cyp1a2 | 5′-ACAACGAGGGACACCTCAC-3′ | 5′-GGGATCTCCCCAATGCAC-3′ |
Actb | 5′-CATCCGTAAAGACCTCTATGCC-3′ | 5′-ACGCAGCTCAGTAACAGTCC-3′ |
Note. The probes for the mouse Cyp1a1 and Cyp1a2 genes detect CYP1A1 and CYP1A2 mRNA, respectively. The probe for the mouse β-actin (Actb) gene detects ACTB mRNA.