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. 2010 Oct;91(5):436–444. doi: 10.1111/j.1365-2613.2010.00720.x

Figure 3.

Figure 3

Activation of SDF-1α up-regulated CXCR4 in VSMCs. (a) Neutralizing antibody of SDF-1α abolished high glucose (HG)-induced CXCR4 expression in VSMCs, as assessed by immunofluorescence with TRITC-conjugated anti-rabbit IgG and counterstaining with Hoechst 33258. Bars = 50 μm. siRNA targeting SDF-1α attenuated HG-induced SDF-1 and CXCR4 expression in VSMCs, while administration of recombinant SDF-1a (100 ng/ml) rescued the SDF-1α siRNA-induced decrease of CXCR4 expression, as assessed by qRT-PCR (b) and Western blotting (c). ELISA results demonstrated that siRNA attenuated HG-induced SDF-1 expression in cell homogenates (d). Results are mean ± SEM of three independent experiments. *P < 0.05, ΔP < 0.05 when vs. NG or siRNA + HG. NC: negative control siRNA.