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. 2010 Oct;91(5):436–444. doi: 10.1111/j.1365-2613.2010.00720.x

Figure 6.

Figure 6

Expression of phenotypic markers in VSMCs. (a) SM22α, OPN and PCNA expressed in thoracic arterial samples from the control (normoglycaemia) and hyperglycaemic rats by immunohistochemical staining. Non-immune IgGs were used as negative control. n = 10 per groups. Bars = 100 μm. mRNA of SM22α, OPN, PCNA and myocardin were assessed by semiquantitative RT-PCR on VSMCs in NG or HG (b) and then the mRNAs expression was normalized to β-actin (c). Protein of SM22α, OPN, PCNA and myocardin were assessed by western blotting on VSMCs in NG or HG (d) and then the protein expression was normalized to β-actin (e). Expression of SM22α, OPN, PCNA and myocardin mRNA was examined in SDF-1α/CXCR4 signalling blockaded VSMCs exposed to HG by administration of AMD3100 (AMD, 10 μM) or SDF-1α neutralizing antibody (Ab, 1 μg/ml) (f and g). Results are mean ± SEM of four independent experiments. *P < 0.05 vs. two associated groups.