Mice received CP-751,871 (0.5 mg/mouse) twice weekly, rapamycin daily 5-days per week or the combination. Tumors were harvested 1 hr after the last dose of rapamycin, and snap-frozen in liquid N
2. Extracts were prepared from tumors and VEGF determined by a human-specific ELISA as described in Experimental Procedures. For each condition (control, CP-751,871, rapamycin or the combination) 6 to 9 independent determinations were used at each time point. * indicates significantly different from controls (P<0.05).
EWS xenografts.
OS xenografts.