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. Author manuscript; available in PMC: 2010 Dec 18.
Published in final edited form as: Cancer Res. 2009 Sep 29;69(19):7662–7671. doi: 10.1158/0008-5472.CAN-09-1693

Figure 6.

Figure 6

VEGF gene expression in control and CP-751,871-treated tumor xenografts. Total RNA was extracted from the tumors 25 hr after administration of CP-751871, reverse transcribed to cDNA and further quantified by real-time RT-PCR as described in Experimental Procedures. The quantity of cDNA in each reaction was normalized to GAPDH and calculated as a ratio of sample cDNA to GAPDH cDNA. Each column represents an average value for duplicate determinations from 3 independent tumors (± SEM). Sample values were plotted in the histogram as per cent of control tumors (untreated). Control levels being set to 100% for each tumor line.