Figure 2.

Activation of the Wnt/β-catenin pathway increases reprograming but not spontaneous cell fusion. (A): Schematic representation of the experimental plan. ESCs without and with treatment with Wnt3a or BIO were DiD stained and cocultured with DiO-stained NS-Oct4-puro cells. The cells were then divided for FACS or reprograming determinations. (B): ES cells pretreated with BIO. Quantification of FACS analysis (% total double-labeled cells) and representative growth plates with quantification of reprograming efficiency (fold-increase in colony number) of the cocultured cells (mean ± SEM; n = 3). (C): Quantification of FACS analysis and reprograming efficiency, with representative growth plates, when ES cells were preatreated with Wnt3a (mean ± SEM; n = 3). Abbreviations: BIO, 6-bromoindirubin-3′-oxime; ES, embryonic stem; FACS, fluorescence activated cell sorting; NS, neural stem.