Skip to main content
. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Alcohol. 2010 Sep 16;45(1):19–32. doi: 10.1016/j.alcohol.2010.07.010

Figure 1.

Figure 1

Schematic of experimental designs. Boxes indicate surgical injection days; bars above indicate training days; shaded areas indicate testing days. (A) This experiment assessed the effects of 5-HT1B receptor overexpression on the initiation of ethanol drinking. Viral vectors were injected on day 1. Animals had forced ethanol exposure (6% and 12%) on days 2–4 and free-choice drinking (water, 6%, and 12%) on days 5–11. (B) This experiment determined the effect of 5-HT1B receptor overexpression on maintenance of ethanol drinking. Animals had forced ethanol exposure (6% and 12%) on days 1–3. Viral vectors were injected on day 4. Days 5–10 were free choice drinking (water, 6%, and 12%). (C) This experiment assessed the effects of 5-HT1B receptor overexpression on sucrose solution intake. Animals were drink trained on days 1–3. Viral vectors were injected on day 4. Drinking preference was tested on days 5–9. (D) This experiment assessed the effects of 5-HT1B receptor overexpression on saccharin solution intake. Animals were trained on days 1 and 2. Virus was injected on day 3. Animals rested on days 4–6. Drinking preference was tested on day 7. (E) This experiment assessed 5-HT1B receptor overexpression on baseline locomotion. Viral vectors were injected on day 1 and locomotor behavior was recorded on days 2–5.