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. Author manuscript; available in PMC: 2012 Jan 11.
Published in final edited form as: Toxicology. 2010 Oct 8;279(1-3):54–64. doi: 10.1016/j.tox.2010.09.007

Figure 4. Changes in gene expression correlate with modification in total protein expression.

Figure 4

(A) Analysis of microarray data revealed changes in mRNA expression following DEHP treatment (50 μg/mL). Integrin α1 (INTGA1), integrin β1 (INTGB1), kinesin 2C (KIF2C), kinesin 11 (KIF11), kinesin 20a (KIF20A), tubulin α1α (TUBBA1A), tubulin β3 (TUBB3), transforming growth factor beta 2 (TGFB2) mRNA expression was decreased following DEHP treatment. (B) Western blot analysis was performed on control and DEHP-treated (50 μg/mL) cardiomyocytes to verify that differential gene expression correlated with protein expression. Samples were quantitated and normalized to GAPDH. Control samples significantly overexpress α1-Integrin, β1-Integrin, α-Tubulin, TGFβ2 and total kinesin relative to DEHP samples. (C) Immunocytochemistry of control and DEHP-treated (50 μg/mL) cardiomyocytes showed less staining for TGFβ2 (left), α-Tubulin (middle), and kinesin 20a (right) in DEHP samples. Images were acquired using a Zeiss LSM 510 confocal system. (Nuclei = blue, size bar = 20 μm) (*p < 0.05).