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. Author manuscript; available in PMC: 2011 May 18.
Published in final edited form as: Dev Cell. 2010 May 18;18(5):802–813. doi: 10.1016/j.devcel.2010.03.014

Figure 2.

Figure 2

Incomplete MOMP is cell-type and caspase independent. (A) MCF-7 cells expressing Smac-GFP were treated with UV (18mJ/cm2) plus Q-VD-OPh (20μM) or TNF (10ng/ml) and imaged every 10 minutes by live-cell confocal microscopy. Representative confocal micrographs pre- and post-MOMP are shown. (B) MCF-7 cells expressing Smac-GFP and matrix-targeted mCherry were treated with ActD (1μM) plus Q-VD-OPh (20μM) and imaged every 10 minutes by live-cell confocal microscopy. Representative confocal micrographs pre- and post-MOMP are shown. Line scans indicate co-localization of Smac-GFP and mito dsRed and correlate to the line drawn in the images. (C) MCF-7 cells expressing Smac-GFP and Omi-mCherry were treated with ActD (1μM) plus Q-VD-OPh (20μM) and imaged every 10 minutes by live-cell confocal microscopy. Representative confocal micrographs pre- and post-MOMP are shown and arrows denote cells undergoing iMOMP. (D) HeLa cells expressing Smac-GFP were treated with TNF (10ng/ml) in the presence of propidium iodide (1μg/ml) and imaged every 10 minutes by live-cell confocal microscopy. Representative confocal micrographs pre- and post-MOMP are shown. Scale bars represent 10μm. See also Figure S2.