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. 2011 Jan;17(1):145–154. doi: 10.1261/rna.2459611

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FIGURE 5. — Prp16 facilitated splicing of brG and brC pre-mRNA. (A) Splicing was carried out with wild-type (lanes 1,2), ACAC (lanes 3,4), brG (lanes 5,6), or brC (lanes 7,8) pre-mRNA in mock-treated (lanes 1,3,5,7) or Prp16-depleted (lanes 2,4,6,8) extracts. dPrp16: Prp16 depletion; M: mock; d: depletion. (B) Splicing was carried out with wild-type (lanes 1–4), brG (lanes 5–8), or brC (lanes 7,8) pre-mRNA in mock-treated (lanes 1,5,9) or Prp16-depleted (lanes 2–4,6–8,10–12) extracts without (lanes 2,6,10) or with the addition of wild-type (lanes 3,7,11) or D473A mutant (lanes 4,8,12) of Prp16.