FIGURE 1.

Protein–protein and protein–RNA interactions of p14 in the human spliceosome. (A, upper) p14 directly contacts the bulged duplex formed between the pre-mRNA branch sequence and U2 snRNA; (lower) p14 as a constituent of the U2 snRNP-associated SF3b particle. (B, upper) Ribbon diagram showing the p14•SF3b155 peptide complex based on the X-ray structure (Schellenberg et al. 2006). (Yellow) p14; (blue) the SF3b155 peptide; (green) Y22. (Lower) Surface representation of the p14•SF3b155 peptide complex showing Y22 exposed within the surface pocket surrounded by conserved basic residues; (light gray) p14; (dark gray) SF3b155 peptide; (yellow) Y22; and (blue) R24, R57, R96, and K100.