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DEER analysis of the RNA double mutants s4U12/s4U21, s4U12/s4U56, s4U42/s4U56, and C′14/A′41 in the absence (black) and in the presence of Tc (gray). (Left) Background-corrected dipolar evolution data F(t). Tick marks are separated by 0.02. (Right) Distance distributions P(r) obtained using DeerAnalysis2006 (Jeschke et al. 2006). In the dotted range, intermolecular spin–spin interaction may contribute.
