FIGURE 4.

Sso-RNase J displays an enhanced degradation rate of mono-phosphorylated than of tri-phosphorylated mRNA. The graph shows the remaining RNA at the indicated times upon degradation with Sso-RNase J. The values are an average of three independent experiments. The values are derived from RNase assays as shown in Figure 3B. (Black bars) 5′-OH-GGA*-″-3′ RNA; (white bars) 5′-P-GGA*-″-3′ RNA; (gray bars) 5′-PPP-GGA*-″-3′ RNA.