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. 2011 Jan;17(1):99–107. doi: 10.1261/rna.2418211

FIGURE 6.

FIGURE 6.

The a/eIF2(γ)-subunit protects RNA from degradation by Sso-RNase J. (A) 5′-PPP-GGA*-″-3′ RNA (0.25 μM) was incubated for 0 to 60 min at 65°C in the presence of Sso-RNase J in the absence (lanes 1–5) or presence (lanes 6–10) of pre-bound a/eIF2(γ) (0.5 μM) or in the presence of a/eIF2(α) (0.5 μM) (lanes 11–15). (B) 5′-P-GGA*-″-3′ RNA was incubated for 0 to 60 min at 65°C in the presence of Sso-RNase J in the absence (lanes 1–5) or presence (lanes 6–10) of a/eIF2γ. The filled arrow indicates the full-length RNA and the open arrow the single A nucleotide.