Skip to main content
. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: J Comp Neurol. 2010 Nov 1;518(21):4375–4394. doi: 10.1002/cne.22462

Figure 8.

Figure 8

Evaluation of TIP39 diffusion and dependence of p-CREB-ir expression in the PVN on injector placement. A–C show p-CREB immunolabeling. D–F show PTH2R immunolabeling of sections adjacent to the upper row sections. A and D show injector placement outside the PVN area expressing PTH2R-ir. B and E show injector placement at the border of the PVN area expressing PTH2R-ir. C and F show injector placement into the medial part of the PVN area expressing PTH2R-ir. Insets in A–C are high-magnification images of p-CREB-ir taken from the areas outlined by the small squares. The arrows in A–C connect the end point of injector tracks to the areas of p-CREB-ir expression shown in the inserts and are markers for the effective distance of TIP39 spread 15 minutes after infusion of 0.5 μl. G is a matched atlas plate (Franklin and Paxinos, 2008) of the brain area shown in the first six panels. Asterisks indicate the placement of injector tips with the corresponding panels indicated by letters. H shows the area covered by fluorescent labeled TIP39 (TIP39-FC) 15 minutes after the infusion of 0.5 μl into the PVN, as determined by plotting the gray value against the distance. The asterisks mark the injector tip and the peak intensity value. The arrowhead indicates a point of 50% decrease in signal intensity, and the number symbol indicates a point of 90% decrease in signal. I is a brightfield image of the field shown in H with visible anatomical landmarks. J plots gray value vs. distance along the white line in H, used to estimate TIP39 diffusion. 3V, Third ventricle; AHP, anterior hypothalamic area, posterior; CM, central medial thalamic nucleus; f, fornix; ic, internal capsule; mt, mammilothalamic tract; MeA, medial amygdala; opt, optic tract; PaPo, paraventricular hypothalamic nucleus, posterior; Pe, periventricular hypothalamic nucleus; PLH, peduncular part of the lateral hypothalamus; Re, reuniens thalamic nucleus; Sub, submedius thalamic nucleus; VM, ventromedial thalamic nucleus; VMH, ventromedial hypothalamic nucleus; VL, ventrolateral thalamic nucleus; ZI, zona incerrta. Scale bar = 0.5 mm.