TABLE 1.
Antibodies Used for Immunohistochemistry
| Antigen | Immunogen | Source | Dilution |
|---|---|---|---|
| Arginine-vasopressin specific neurophysin |
Rat posterior pituitary extract, keyhole limpet hemocyanin (KLH) coupled |
Dr. Harold Gainer, NIH; mouse monocolonal PS41 |
1:1,000 |
| β-Galactosidase | Purified full-length native protein | AbCam, Cambridge, MA; chicken No. AB9361 |
1:15,000 |
| Corticotrophin releasing-hormone |
Synthetic peptide corresponding to full-length rat/human CRF(1–41) |
Dr. Wylie Vale, Salk Institute; rabbit polyclonal PBL rC70 pool 397-223 |
1:5,000 |
| Fluorogold | Fluorogold (hydroxystilbamidine) | Chemicon; rabbit polyclonal AB153 | 1:1,000 |
| Glutamate | Glutamate-glutaraldehyde conjugate | Chemicon; rabbit polyclonal AB5018C | 1:1,000 |
| Oxytocin-specific neurophysin |
Rat posterior pituitary extract, KLH coupled |
Dr. Harold Gainer, NIH; mouse monocolonal PS38 |
1:1,000 |
| pCREB | Synthetic phosphopeptide CSRRPsYRKI, where s represents phospo-Ser133 of human CREB, coupled to KLH |
Cell Signaling, Danvers, MA; rbbit monoclonal catalog 9198L |
1:1,000 |
| PTH2-R | Synthetic peptide corresponding to residues 480–500 of the rat PTH2R (GenBank U55836), KLH coupled |
Dr. T. Usdin, NIH; rabbit polyclonal 2710 |
1:20,000, 1:5,000 |
| Somatostatin | Synthetic 1–14 cyclic somatostatin, thyroglobulin coupled |
Chemicon; rat monoclonal catalog MAB354 |
1:1,000 |
| Thyrotropin releasing-hormone |
Prepro-TRH240–255, bovine thyroglobulin coupled |
Dr. Eduardo Nillni, Brown University; rabbit polyclonal pYE17 |
1:1,000 |
| TIP39 | Full-length TIP39, KLH coupled | Dr. T. Usdin, NIH; rabbit polyclonal pooled 7250, 7251 |
1:3,000 |
| Tyrosine hydroxylase | Tyrosine hydroxylase purified from PC12 cells |
Chemicon; mouse monoclonal catalog MAB358 |
1:1,000 |
| VGlut2 | GST fusion protein containing AA 519–582 of rat VGlut2 |
Dr. Robert Edwards, UCSF; rabbit polycolonal |
1:10,000 |