Skip to main content
. 2010 Dec;30(12):893–900. doi: 10.1089/jir.2010.0046

FIG. 1.

FIG. 1.

Effects of lipopolysaccharide (LPS) and interferon (IFN)γ on Ets mRNA expression in primary monocytes. (A) Primary human monocytes were stimulated with 100 ng/mL of LPS with or without IFNγ pretreatment (100 U/mL, added 3 h before LPS) for 3 h. (B) Gene expression in primary human monocytes transfected with control or Tel-2-specific short interfering RNAs. (C) THP-1 cells were transduced with lentiviral particles expressing enhanced green fluorescent protein (eGFP) or epithelium-specific Ets factor 3 (ESE-3) and stimulated with 100 ng/mL of LPS with or without IFNγ pretreatment (100 U/mL, added 3 h before LPS) for 3 h. mRNA levels were analyzed using real-time PCR and normalized relative to β-actin; results are expressed as mean ± SD of triplicate determinants. Ets, E26 transformation-specific; MMP, matrix metalloprotease; IL, interleukin; FLI-1, Friend leukemia virus integration 1.