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Modulation of FLI-1 expression in THP-1 monocytic cells. THP-1 cells were transduced with lentiviral particles expressing eGFP or FLI-1 and stimulated with LPS with or without IFNγ pretreatment, and FLI-1 mRNA was measured using real-time PCR. mRNA levels were normalized relative to β-actin, and real-time PCR results are expressed as mean ± SD of triplicate determinants.
