FIG. 3.

FLI-1 overexpression enhances LPS-induced MMP expression but variably affects cytokine production. (A–C) THP-1 cells were transduced with lentiviral particles expressing eGFP or FLI-1 and stimulated with 100 ng/mL of LPS with or without IFNγ pretreatment (100 U/mL, added 3 h before LPS) for 3 h. mRNA levels were analyzed using real-time PCR and normalized relative to β-actin; results are expressed as mean ± SD of triplicate determinants. TNF, tumor necrosis factor.