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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Int J Oncol. 2011 Jan;38(1):249–256.

Figure 1.

Figure 1

Akt phosphorylation during the development of acquired TRAIL resistance and during TRAIL treatment. (A) DU-145 cells were treated with TRAIL (200 ng/ml) for 4 h. Detached cells were then removed by washing with PBS, followed by the addition of fresh media to the remaining attached cells, which were then incubated for the indicated times (0, 1, 2 days). Cells were lysed, and lysates were analyzed for phosphorylated Akt, and Akt. Actin was used to confirm equal amount of proteins loaded into each lane. Lane 1, control; lane 2, attached cells after 0 day of TRAIL; lane 3, attached cells after 1 day of TRAIL; lane 4, attached cells after 2 days of TRAIL (B) DU-145 cells were treated with 200 ng/ml TRAIL for various times (0–4 h). Cells were lysed, and lysates were analyzed for the presence of PARP, phosphorylated Akt and Akt. Actin was used to confirm that equal amounts of protein were loaded into each lane.