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. Author manuscript; available in PMC: 2011 May 21.
Published in final edited form as: ACS Chem Biol. 2010 May 21;5(5):499–506. doi: 10.1021/cb9003207

Figure 2.

Figure 2

Purification of HCE-Gly52R ATCase. SDS PAGE (a) and nondenaturing PAGE (b) visualized by coomassie blue stain (a and b, left) or fluorescence (a and b, right) of the purification of the HCE-Gly52R ATCase. Lanes 1, wild-type ATCase; Lanes 2, E. coli ATCase c3 containing a C-terminal His tag; Lanes 3, total soluble protein from cell lysate; Lanes 4, HCE-Gly52R ATCase after column purification employing Chelating Sepharose Fast Flow (GE Healthcare) charged with Ni2+; Lanes 5, HCE-Gly52R ATCase after column purification employing Phenyl Sepharose (GE Healthcare).