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. Author manuscript; available in PMC: 2011 May 21.
Published in final edited form as: ACS Chem Biol. 2010 May 21;5(5):499–506. doi: 10.1021/cb9003207

Figure 4.

Figure 4

ATP and CTP binding to HCE-Gly52R ATCase and the isolated regulatory subunit. a) Alteration in the fluorescence of the HCE-Gly52R ATCase upon the binding of ATP (green) and CTP (red) at pH 7.0 (squares) and 8.3 (circles). 50 μg ml−1 of HCE-Gly52R ATCase in 50 mM Tris-acetate buffer at pH 8.3 or 20 mM Bis-Tris, 20mM Tris, 20mM CAPS buffer at pH 7.0 at 25 °C was excited at 360 or 330 nm respectively. The fluorescence intensity at 455 nm was normalized to the first data point (0 mM NTP) and plotted versus the nucleotide concentration. b) Alteration in the fluorescence of the HCE-Gly52R r2 species upon the binding of ATP (green) and CTP (red) at pH 8.3. Conditions similar to those part a.